Performing Western and Dot Blots on the Q-View Imager
While the Q-View Imager was exclusively designed and developed to be used with Q-Plex Arrays, some customers have been successful in using the device for western and dot blot imaging. Due to the numerous methods and reagents available, we are not able to provide specific steps on how to do this. Keep in mind, customers will have to optimize their methods to the imager. Notwithstanding, we can provide a few tips so if you do choose to use the Q-View Imager for this purpose, you’ll have a better chance of success.
There are numerous chemiluminescent substrates available on the market of a variety of quality and performance. The substrate used in the Q-Plex kits is very sensitive and stable. The customers that have had good success with the Q-View Imager have used this substrate. From what we have observed, some of the less sensitive substrates are not acceptable for this application. Please contact your sales representative to learn more about where you can purchase this substrate.
The camera used in the Q-View Imager is able to do long exposures. We recommend doing multiple exposures, some short and some longer. It may be necessary to do exposures up to five minutes or longer.
Streptavidin HRP (SHRP)
While every experiment is different we recommend maximizing the concentration of SHRP to achieve the desired sensitivity.